Absorption, thermolysin

Resistance to peptidases was also reported for the octapeptoid 6.106 when incubated with papain, chymotrypsin, or thermolysin [229], However, resistance to peptidases may not be synonymous with a long half-life in vivo, since many factors beside peptidases can be expected to contribute to the elimination of peptoids. An indirect indication of this effect can be found for antimicrobial peptoids and particularly compound CHIR29498 (6.107) [232], In mice infected with Staphyllococus aureus, this peptoid was less active when injected 2 h post-infection compared to 0 h or 0.5 h. The conclusion drawn by the authors was that the compound requires optimization for improved absorption or stability within the body. 

Fig. 6. Absorption and magnetic circular dichroism spectra of Co2+ thermolysin. (A) MCD spectra. (B) Absorption spectra.

The complexes CoL (L = carboxilate Iz = imidazole) have visible and MCD spectra similar to cobalt thermolysine and cobalt carboxipeptidase A98. The g values for (Co(CH3COO)2Iz2) are almost axial, while a relatively large rhombic splitting is apparent for (Co(C2H5COO)2Iz2). The molar absorption coefficients of these complexes in ethanol are very small for tetrahedral complexes, but the possibility of forming octahedral species cannot be excluded. 

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