Absorption spectrum of Calcofluor free in PBS buffer

1 Plot the baseline from 190 to 450 nm, using PBS buffer in both absorbance cuvettes (buffer vs. buffer). Both cuvettes contain 1 ml of PBS. 

2 To the sample cuvette, add with a pipette Pasteur very small quantity of Calcofluor White powder, then mix slowly. 

3 Plot absorption spectrum of Calcofluor White from 190 to 450 nm. 

4 Prepare a new cuvette with PBS buffer and repeat step 1. 

5 Dissolve in the new sample cuvette small quantity of lyophilized a i -acid glycoprotein. Plot protein absorption spectrum from 200 to 400 nm. 

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