Ascorbate oxidase absorption spectrum

Fig. 5-14. The visible absorption spectrum of ascorbate oxidase (curve 1) and in the presence of a substrate AH2 (curve 2). Reproduced with permission from Dawson (1960).

The reoxidation studies on laccase and ascorbate oxidase are listed in Table IX. The reoxidation of the type-1 copper and of the trinuclear copper site occurs at a rate of 5 x 10 M" sec" both for tree laccase 134) and for ascorbate oxidase 135). During reoxidation with H2O2, an 02 " intermediate is formed in several minutes, which is documented for tree laccase by changes in the CD spectrum 136) and for ascorbate oxidase in the formation of an absorption band at 350 nm... 

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