Absence of Strong Acids or Alkalies Mutarotation

A continuously recording polariscope has been developed (46b) which should aid mutarotation measurements. 

The mutarotation of a-glucose may be represented by the equation for a first-order reversible reaction. 

Equation (2) may be integrated and expressed in terms of the optical rotations in the form of equation (3) (47). 

In case the rotations are read on a saccharimeter, the values observed (°S) are multiplied by the factor 0.3462 to give a. 

The rotation varies with the wavelength of the light source, and usually the sodium D line is employed. Most rotations are measured at 20°C. The solvents most commonly employed are water and chloroform. 

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