A first HPLC experiment

Although this beginner s experiment described here is simple, it is recommended that you ask an experienced chromatographer for assistance. [Pg.5]

It is most convenient if a HPLC system with two solvent reservoirs can be used. Use water and acetonitrile both solvents need to be filtered (filter with 1 p,m pores) and degassed. Flush the system with pure acetonitrile, then connect a so-called reversed-phase column (octadecyl ODS or Cig, but an octyl or Cg column can be used as well) [Pg.5]

Practical High-Performance Liquid Chromatography, Fifth edition Veronika R. Meyer [Pg.5]

After you have finished this work, flush the column again with pure acetonitrile. [Pg.7]

Practical High-Performance Liquid Chromatography, Fourth edition Veronika R. Meyer 2004 John Wiley Sons, Ltd ISBN 0-470-09377-3 (Hardback) 0-470-09378-1 (Paperback) [Pg.4]

2 HPLC separation of coffee. Conditions colunnn, 15cnnx2nnnn i.d. stationary phase, YMC 120 ODS-AQ, 3 im nnobile phase, 0.3 ml min water-acetonitrile (8 2) UV detector 272 nm. [Pg.6]

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