A-Acetyltryptophanamide

A report by Lulka and Chambers [27] describes the properties of surface-imprinted silicas using A-acetyltryptophanamide (NATA) or fluorescein (Fig. 20.9) as templates. Silica gel was mixed with bis(2-hydroxyethyl)-aminopropyltriethoxysi-lane (as functional monomer), tetraethoxysilane and the template and the slurry... 

Lulka and co-workers have imprinted two fluorescent compounds, fluorescein (Fig. 2, 6) and A-acetyltryptophanamide (NATA) (Fig. 2,7) with organic silanes bis (2-hydroxyethyl) -aminopropryltriethoxysilane (HAPTES) and tetraethoxysilane (TES) [23]. The binding constants were determined with steady-state fluorescence spectroscopy. It was found that high affinity binding sites with Kj s in the range of sub - to low micromolar were achieved. 

Figure 1 (a) Absorption and fluorescence spectra of fluorescent amino acids blocked with peptide bonds N-acetyltryptophanamide (black),... 

The recently discovered enzyme tryptophane side chain a, 3-oxidase dehydrogenates acetyltryptophanamide and other peptides containing tryptophane. Apart from the fact that no hydrogen peroxide is formed... 

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