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A-Acetolactatedecarboxylase

Revision Classifications and reactions added for a-Acetolactatedecarboxylase Aminopeptidase, Leucine and Lysozyme Heavy Metals (as Pb) specification and determination deleted from Additional Requirements. [Pg.17]

Application and Principle This procedure is used to determine a-acetolactatedecarboxylase (ALDC) activity, expressed as ADU/g. The assay is based on the decarboxylation of a-aceto-lactate to acetoin by a-Acetolactatedecarboxylase. Acetoin is reacted with a mixture of naphthol and creatine to form a red color for which absorbance is measured at 522 ran. The assay is based on a 20-min decarboxylation of the a-acetolactate substrate at 30° and at pH 6.0. [Pg.104]

Calculation One a-acetolactatedecarboxylase unit (ADU/g) is the amount of enzyme that produces 1 pmol of acetoin per min by decarboxylation of a-acetolactate under the given reaction conditions. [Pg.104]

See other pages where A-Acetolactatedecarboxylase is mentioned: [Pg.148]    [Pg.150]    [Pg.19]    [Pg.21]    [Pg.104]    [Pg.111]    [Pg.111]    [Pg.112]    [Pg.117]    [Pg.117]    [Pg.148]    [Pg.150]    [Pg.19]    [Pg.21]    [Pg.104]    [Pg.111]    [Pg.111]    [Pg.112]    [Pg.117]    [Pg.117]   
See also in sourсe #XX -- [ Pg.148 , Pg.150 ]



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