3H-Galactose

Seyama, Y., Kalckar, H. M. Specific tritium labeling of uridine diphosphogalactose 4-epimerase by d-1-3H Galactose. Biochemistry 11, 36—39 (1972). 

Monolayers of normal and FH cultured fibroblasts were incubated in the presence of [3h] - galactose in either LPDS medium or medium containing FCS. The medium was then collected and dialyzed at 4°C for 24 hrs against 4 changes of 4L water. Antibody against human serum was then added to the culture medium. Following incubation for 48 hrs at 4° the samples were centrifuged at 30,000 x g for 30 min at 4°. The supernatants were carefully withdrawn and the pellets rinsed with ice cold PBS and frozen until further use. The supernatants were freeze-dried. 

Incorporation of Radioactivity Derived from [3H] Galactose into the Individual Glycosphingolipids of Normal and Familial Hypercholesterolemic Homozygous (FH) Cells Cultured in Fetal Bovine Serum... 

G. P. Akulov, N. A. Korsakova, and B. K. Kudelin, Use of kinetic isotope effects in enzymatic oxidation of D-[6-3H]galactose for increasing its molar activity, J. Labelled Comp. Radiopharm., 27 (1989) 803-810. 

---