3D STORM

To demonstrate that 3D STORM can resolve the 3D morphology of nanoscopic structures in cells, we imaged clathrin-coated pits using a direct immunofluorescence scheme. The clathrin in the cell was stained with... 

Fig. 20.7. 3D STORM imaging of microtubules in a cell, (a) Conventional immunofluorescence image of microtubules in an area of a cell, (b) The 3D STORM image of the same area with the 2-position information color-coded according to the colored scale bar (c) The x — y, x — z, and y — z cross-sections of a small region of the cell outlined by the white box in (b)...

B. Huang, S.A. Jones, B. Brandenberg, X. Zhuang, Whole-ceU 3D STORM reveals interactions between cellular structures with nanometer-scale resolution. Nat. Meth. 5, 1047-1082 (2008)... 

Extensive, thin storm-deposited silty-sandy sheets interbedded with fine-grained marls occur in areas distal to the tidal delta (Boc)c, 1989). Alternating storm beds and silty fossiliferous marls, both intensely bioturbated, form rhythmically bedded successions (Fig. 3D). The grain size of the coarser-grained beds decreases in a distal direction (Bock, 1989). 

Stochastic optical reconstruction microscopy (STORM) and direct STORM (dSTORM), also referred to as ground-state depletion and single-molecule return (GSDIM), use fluorochromes that reversibly cycle between fluorescent (ON) and dark (OFF) states upon exposure to light of specific wavelengths [158, 162-164]. STORM-based ICC (Fig. 3D) relies on the proximity of two fluorochromes attached to an antibody in a specific ratio and at a specific distance (Fig. 3E), whereas dSTORM (Fig. 3E) employs a... 

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