SEARCH Articles Figures Tables Conjugation of the biological peptide -enkephalin to BSA using EDC. The graph shows the gel filtration profile after completion of the conjugation reaction. A blank run with no added EDC was done to illustrate the peak absorbance that would be obtained if no conjugation took place. With addition of 10 mg of EDC to a reaction mixture consisting of 2 mg of BSA plus 2 mg of peptide, nearly complete conjugate formation was obtained. [Publishers Imprint] See Also: Conjugation of the biological peptide -enkephalin to BSA using EDC. The graph shows the gel filtration profile after completion of the conjugation reaction. A blank run with no added EDC was done to illustrate the peak absorbance that would be obtained if no conjugation took place. With addition of 10 mg of EDC to a reaction mixture consisting of 2 mg of BSA plus 2 mg of peptide, nearly complete conjugate formation was obtained. Conjugation of the biological peptide -enkephalin to BSA using EDC. The graph shows the gel filtration profile after completion of the conjugation reaction. A blank run with no added EDC was done to illustrate the peak absorbance that would be obtained if no conjugation took place. With addition of 10 mg of EDC to a reaction mixture consisting of 2 mg of BSA plus 2 mg of peptide, nearly complete conjugate formation was obtained.