Yphantis High-Speed Method

3 Yphantis (High-Speed) Method (Rotor Velocity 28,000-32,000 rpm) 

The crucial part in analyzing the data is the reading of r versus y of the curve in the photographic plate. The enlarged curve is in the following form  

With the data of y, yo, and r now available, we plot the final results  

The experimental points are expected to be on a straight line and the slope is equal to [d In(y — yo)]/dr. From the slope of the plot we can thus calculate molecular weight. 

The following table (van Holde, 1967) compares the low-speed method to the high-speed method  

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