Yeast lysis structured model

The two models of yeast lysis presented here have been developed to serve two different purposes. The simple model is a lumped, two-step model which follows the major features of the data and may prove useful for design of lysis reactors. The structured model, which can account for the source of protein within the cell, was developed to gain a mechanistic basis for predicting the effects of untested process conditions, and to aid insight into the physical processes at work during lysis. 

The simple and structured model simulations for yeast mass and soluble protein, peptides and carbohydrates are compared in Figure 6 for the yeast and enzyme concentration shown in Figures 3 and 4, and in Figure 7 for a concentrated yeast cell slurry. The simple model fits the data fairly well at both yeast concentrations, in every variable except the peptides. The fit for all variables at longer reaction times is directly related to use of the extent-of-reaction term Y , in the yeast lysis equation. 

Figure 5 Structured model simulation of yeast lysis 5a Cell structures...

Structured and Simple Models of Enzymatic Lysis and Disruption of Yeast Cells... 

Hunter, J.B. and Asenjo, J.A., "A structured, mechanistic model of the kinetics of enzymatic lysis and disruption of yeast cells", Biotechnol. Bioeng., 1986 (being submitted). 

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