Xylose chromatographic analysis

Chromatographic Analysis of Wood Sugars. This analysis requires acid hydrolysis of the polysaccharide to yield a solution mixture of the five wood sugar monomers, i.e., glucose, xylose, galactose, arabinose, and mannose. The solution is neutralized, filtered. 

Fig. 11.4.6. Analysis of a carbohydrate mixture on an amine modified silica column. Chromatographic conditions column. Waters Sugar-Pak I mobile phase, 10 M calcium acetate flow rate, 0.5 ml/min temperature, 90 °C. Peaks 1, ethanol 2, ethylene glycol 3, glycerol 5, xylose 6, fructose 7, galactose 8, glucose 9, mannitol 10, sorbitol 11, lactose 12, maltose 13, sucrose 14, melezitose m, artifact due to mobile phase. Reproduced from Baust et al. (1983), with permission.

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