Wide deconvolution procedure

Since the development of the Fourier-transform deconvolution procedure for OHD-RIKES data by McMorrow and Lotshaw (22), the intermolecular dynamics of a wide range of liquids have been studied with this technique (26,52,65-85). Figure 13 illustrates representative OKE reduced spectral densities we have recorded in symmetric-top liquids, including acetonitrile, benzene, benzene-d6, carbon disulfide, chloroform, hexafluorobenzene, mesitylene, and 1,3,5-trifluorobenzene. Although there are conspicuous differences among these spectra, they are all broad and relatively featureless. Indeed, with rare exceptions the reduced spectral densities of simple liquids are devoid of sharp features, which makes it difficult to find an unambiguous interpretation of these spectra. 

Quantitative accuracy and precision (see Section 2.5 below) often depend upon the selectivity of the detector because of the presence of background and/or co-eluted materials. The most widely used detector for HPLC, the UV detector, does not have such selectivity as it normally gives rise to relatively broad signals, and if more than one component is present, these overlap and deconvolution is difficult. The related technique of fluorescence has more selectivity, since both absorption and emission wavelengths are utilized, but is only applicable to a limited number of analytes, even when derivatization procedures are used. 

With metastable ions, the isotope effect Ij/In is obtained from metastable ion abundances. The abundance is given by the area under the metastable peaks after the peak has been deconconvoluted to remove the broadening effect of finite slit widths and other factors. The usual procedures adopted in practice are either to work with narrow slits, determine peak areas and neglect deconvolution, or to work with wide slits and measure peak heights. 

Both regions of the semicrystalline framework contribute to the XRD pattern of a polymeric sample crystalline domains originate sharp reflections, whereas the amorphous zones produce a wide and diffused halo. A fitting procedure for the experimental patterns can be performed, by which the contributions of the crystalline and amorphous domains are deconvoluted [92] (Figure 4.3). 

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