Whooping cough

Pertussis toxin Gj,0 proteins ADP-ribosylation Inhibition ofG protein signaling (whooping cough)... 

Soelaiman S, Wei BQ, Bergson P, Lee YS, Shen Y, Mrksich M, Shoichet BK, Tang WJ. Structure-based inhibitor discovery against adenylyl cyclase toxins from pathogenic bacteria that cause anthrax and whooping cough. J Biol Chem 2003 278 25990-7. 

Bordetella pertussis is the causal organism of whooping-cough, a disease which... 

Killing. The proeess by which the live bacteria in the culture are killed and thus rendered harmless. Heat and disinfectants are employed. Heat and/or formalin are required to kill the cells of Bordetella pertussis used to make whooping-cough vaccines, and phenol is used to kill the Vibrio cholerae in cholera vaccine and the Salmonella typhi in typhoid vaeeine. 

Whooping-cough (Pertussis) (aceiiuiar)t Cultures of Bord. pertussis 1 Harvest 2 Extraction and blending of cell components As for whole-cell whooping-cough vaccine Weight gain test in mice to exclude excess toxicity... 

Notes Diphtheria and whooping cough vaccines are seldom used as single-component preparations but as components of diphtheria/tetanus vaccines and diphtheria/tetanus/pertussis vaccines. A combined diphtheria/tetanus/pertussis/Hib vaccine is available. 

Public confidence in the safely of vaccines and immunization procedures is essential if compliance is to match the needs the community. In this respect public concern and anxiety, in the mid 1970s, over the peroeived safety of pertussis vaccine led to a reduction in coverage of the target group from ca. 80% to ca. 30%. Major epidemics of whooping-cough, with over 100000 notified cases, followed in 1977/1979 and 1981/83. By 1992, public confidence had returned, coverage had increased to 92% and there were only 4091 reported cases. 

This material is hazardous through inhalation and penetration through broken skin. Specific signs and symptoms of exposure to this toxin have not been established or have not been published. However, the major symptoms associated with whooping cough, the disease caused by Bordetella pertussis, are related to the effects of this toxin. 

It seems likely that many such (recombinant) subunit vaccines will gain future regulatory approval. One such example is that of Bordetella pertussis subunit vaccine. B. pertussis is a Gramnegative coccobacillus. It is transmitted by droplet infection and is the causative agent of the upper respiratory tract infection commonly termed whooping cough . 

Pertussis (whooping cough) DTaP, DTaP/Hib, Tdap Cell based... 

Pertussis toxin is a protein secreted by the bacterium Bordetella pertussis which causes whooping cough. The toxin enters a cell where it also catalyses ADP-ribosylation of the a-subunit of G-protein. 

Pertussis (whooping cough) B. pertussis 40 to 50 mg/kg/day, given in divided doses 5 to 14 days... 

The resulting failure ofG, to inhibit adenylate cyclase increases cyclic AMP in airway cells and leads to fluid imbalance and the severe, life-threatening congestion of whooping cough. 

Other immimizations use substances taken from the microorganism that causes the disease, and some even use whole killed disease-causing bacteria. The DPT shots that children receive before entering school are made up of proteins isolated from the bacteria that cause diphtheria and whooping cough (pertussis), plus killed tetanus bacteria. The pertussis and diphtheria proteins used in the... 

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