Whole cell screens

Reagents. The following buffers and solutions are needed for whole cell screening growth media Dulbecco s modified Eagle s medium (DMEM), 10% fetal calf serum (FCS), 100 units/ml penicillin, and 100 /ig/ml streptomycin, PBS 8 mM Na2HP04, 1.4 mM KH2P04, 137 mM NaCl, 2.7 mM KC1, pH 7.2. 

F. Whole-Cell Screening of Enzyme Inhibitors Lipid A Biosynthesis... 

Ekins S, Freundlich JS (2011) Validating new tuberculosis computational models with public whole cell screening aerobic activity datasets. PharmRes 28 1859-1869... 

The large-scale whole-cell screening efforts have created large training sets for TB models such as the Bayesian models described by us and others. The caveat with these models is they are likely limited to predicting compound activity under the exact in vitro conditions used (15). However, by considering data from different groups, we have shown that this may not be a major issue (21). Hence such models can be used to filter vendor libraries of molecules and natural products (21). 

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