When to Tune the NMR Probe and Calibrate RF Pulses

Many NMR probes now available are designed so that probe tuning response is relatively insensitive to sample changes. Consequently, we do not need to tune our probe or calibrate our RF pulses before carrying out various 1-D and 2-D NMR experiments. A probe of this nature is an essential feature of a high-throughput instrument. 

Not all NMR data sets should be collected using a high-throughput NMR probe, because damping the probe s tuning response sacrifices 

With practice, we will be able to tune two RE channels of an NMR probe in under a minute, but we must take care not to damage the relatively delicate glass capacitors found in NMR probes (some newer NMR probes no longer contain these electrical elements—a point for rejoicing for those charged with maintaining NMR instrumentation in the multiuser environment). 

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