What Is Capillary Electrophoresis

Electrophoresis is the migration of ions in an electric field. Anions are attracted to the anode and cations are attracted to the cathode. Different ions migrate at different speeds. Capillary electrophoresis is a high-resolution separation technique conducted with solutions of ions in a narrow capillary tube. A clever modification of the technique allows us to separate neutral analytes also. Capillary electrophoresis applies with equal ease to the separation of macromolecules, such as proteins and DNA, and small species, such as Na and benzene. Capillary electrophoresis can analyze the contents of a single cell. 

Capillary electrophoresis can provide extremely narrow bands. Three mechanisms of band broadening in chromatography are longitudinal diffusion (B in the van Deemter equation 21-7), the finite rate of mass transfer between the stationary and mobile phases (C in the van Deemter equation), and multiple flow paths around particles (A in the van Deemter equation). An open tubular column in chromatography or electrophoresis reduces band broadening (relative to that of a packed column) by eliminating multiple flow paths (the A term). Capillary electrophoresis further 

Cations are attracted to the negative terminal (the cathode). Anions are attracted to the positive terminal (the anode). 

Background electrolyte, also called run buffer, is the solution in the electrode reservoirs. It controls pH and ionic composition in the capillary. 

The greater the charge on the ion, the faster it migrates in the electric field. The greater the size of the ion, the slower it migrates. 

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What is capillary electrophoresis and what advantages does it have over other conventional electrophoresis techniques. 

WHAT IS CAPILLARY ELECTROPHORESIS AND WHAT ROLE DOES IT HAVE IN TEOA ... 

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