What Are theThree-Dimensional Shapes of Polypeptides and Proteins

Edman degradation cleaves Glu from the pentapeptide therefore, glutamic acid must be the A/-terminal amino acid, and we have 

Fragment A from chymotrypsin-catalyzed hydrolysis contains Phe. Because of the specificity of chymotrypsin, Phe must be the C-terminal amino acid of fragment A. Fragment A also contains Glu, which we already know is the /V-terminal amino acid. From these observations, we conclude that the first three amino acids in the chain must be Glu-His-Phe, and we now write the following partial sequence  

The fact that trypsin cleaves the pentapeptide means that Arg must be within the pentapeptide chain it cannot be the C-terminal amino acid.Therefore, the complete sequence must be 

Deduce the amino acid sequence of an undecapeptide (11 amino acids) from the experimental results shown in the following table  

Experimental Procedure Amino Acids Determined from Procedure 

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What Are theThree-Dimensional Shapes of Polypeptides and Proteins ... 

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