Vitellogenic ovaries

Many endogenous ecdysteroids which can be considered as the intermediates of ecdysone biosynthesis were isolated from the ovaries of L. migratoria by Hoffmann and Horn [204]. From the results of the bioconversion of labelled precursors, the following sequence in the biosynthetic pathway of ecdysteroids in vitellogenic ovaries of the insect was proposed conversion of cholesterol to 3)3-hydroxy-5)8-cholest-7-en-6-one followed by 14a-hydroxylation to 3/S,14-dihydroxy-5/6-cholest-7-en-6-one (112) hydroxylation at C-25 and C-22 (in this order) to 2-deoxyecdysone (114) hydroxylation at C-2 to ecdysone (107). 

The direct effect of an allatostatic ovary-derived factor was first reported in a survey of various tissue extracts of vitellogenic locust females on JH synthesis in vitro (16). We have recently found... 

Fig. 5A -D. Spectra of lipoproteins from hemolymph and ovary of crabs. A Lipoprotein from ovary of crab Cancer pagurus) in 0.2 M phosphate buffer, pH 7. (Zagalsky et al. 1967) B Lipoprotein II (female-specific lipoprotein) isolated from hemolymph of vitellogenic blue crab Callinectes sapidus). Lipoprotein was in 0.05 M TRIS buffer (pH 8.0). (Lee and Puppione 1988). C Ovorverdin from Homarus gammarus in 0.5 M NaCl — 5 mm EDTA (pH 5). (Zagalsky 1985). D Lipoproteins from ovary of scallop, Pecten maximus, in 0.2 M phosphate buffer (pH 7). Free carotenoids were released from the lipoproteins by extraction with acetone. (Zagalsky et al. 1967)...

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