Vesicles size distribution

One way is to label the pre-existing vesicles, and then follow the destiny of the label in the vesicle size distribution. The label that has been used to this aim is ferritin, which has been entrapped into vesicles. Ferritin is an iron-storage protein in plants and mammals, and consists of a hollow protein shell of c. 12 nm containing... 

A more efficient technique is to determine vesicle size distributions from 2D cross-sections of hand samples (Dullien and Dhawan 1973 Russ 1986 Marsh 1988 Mangan 1990 Toramaru 1990). Sample cross-sections can be optically scanned and the data numerically processed to... 

Phospholipid hydrolysis Cholesterol autooxidation Antioxidant degradation Physical stability Vesicle size distribution submicron range... 

Korgel, B. A., van Zanten, J. H., and Monbouquette, H. G. (1998), Vesicle size distributions measured by flow field-flow fractionation coupled with multiangle light scattering, Biophys. J., 74, 3264-3272. 

To obtain the vesicle size distribution we substitute the above expressions into eqn (2.8). For Ri < R y/Q obtain the following distribution law for the concentration of vesicles of radius Ri ... 

Vesicle Size Distribution and zeta-Potential Measurement... 

Figure 3. Freeze-fracture electron microscopy analysis of the vesicle size distribution in the case of the spontaneous vesiculation of oleic acid/oleate. (A) Vesicles formed from the hydrolysis of 25 mM oleic anhydride (overall concentration) at 30 °C, yielding 50 mM oleic acid/oleate. (B) Vesicles extruded throughout 50 nm diameter filters. (C) Vesicles formed upon hydrolyzing 20 mM oleic anhydride (same conditions as in A) in the presence of pre-added extruded vesicles B—all in 0.2 M bicine buffer pH 8.5. For details see ref. 8.

Figure 22.14 Sum curve of the volumetric vesicle size distribution (23) for different total flow rates obtained with the IMM setup at about 80°C and at an almost constant surfactant content of 10wt% [3],...

Skipping a great deal of algebra, it can be shown that the ratio of has a quadratic dependence on /j, tan 9, and [31 ]. Since this quantity is a nontrivial function of these variables, it is difficult to solve. However, by comparing the distribution of vesicle size for a population of vesicles from electron microscopy to that for a reduced radius (radius in units of the above variables) obtained from amperometty, one can isolate values for each vesicle in the amperometric data set. Using parameters contained within amperometric exocytosis data and the distribution of vesicle size from electron microscopy data, a histogram of vesicle size can be calculated directly from the electrochemical data (Figure 11). Anderson et al. have calculated the relative standard deviation of the size distribution calculated from electrochemical data to be 25%, which closely matches the relative standard deviation of the vesicle size distribution measured by electron microscopy [29]. 

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