Vectors replicators

The first transient expression system for cytochrome P450 expression was the COS cell system which couples monkey cells with an SV40-based expression vector (Zuber et al., 1986). The cDNA of interest is introduced into a plasmid expression vector under control of a heterologous promoter. The vector is introduced into COS cells via electroporation or another method allowing DNA uptake. The plasmid vector replicates within the cells and cDNA-derived protein accumulates within the cell. 

Boris-Lawrie, K. and Temin, H.M. (1994) The retroviral vector replication cycle and safety considerations for retrovirus-mediated gene therapy. Ann. N.Y. Acad. Sci., 716, 59-70. 

Vieira HLA, Estevao C, Roldao A, Peixoto C, Sousa M, Cruz PE, Carrondo MJT, Alves PM (2005), Triple layered rotavirus VLP assembly kinetics of vector replication, mRNA stability and recombinant protein production, J. Biotechnol. 120 72-82. 

SV40 to high copy number with deletions in the early region, SV40 vector replication reaching a maximum after about 2 days. 

Nuclease Large DNA fragments Insert in Bacterial 100-300 kb BAG vector replication"... 

Fig. 3 Delivery systems used in clinical gene transfer. During gene therapy, an expression vector (therapeutic gene) is transferred to somatic cells via a delivery system, for example, a viral or nonviral vector (replication-incompetent), a naked nucleic acid, or a recombinant, mostly conditionally replication-competent microorganism. The gene...

Maroteaux, L., Kahana, C., Mory, Y., Groner, Y., and Revel, M., 1983, Sequences involved in the regulated expression of the human interferon-pi gene in recombinant SV40 DNA vectors replicating in monkey cells, EMBO J. 2 325-332. 

YEp Vectors The YEp yeast episomal plasmid vectors replicate autonomously because of the presence of a segment of the yeast 2 pm plasmid that serves as an origin of replication (2 pm oii). The 2 pm ori is responsible for the high copy-number and high frequency of transformation of YEp vectors. 

Boris-Lawrie K, Temin HM. The retroviral vector. Replication cycle and safety considerations for retrovims-mediated gene therapy. Ann NY Acad Sci 1994 716 59-70 discussion 71. 

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