UV absorbers, determination

The same considerations will apply to other nonspecific methods of detection, such as fluorescence or UV absorbance determinations. Particularly with these methods, it must be appreciated that many of the cells used to form mono-layers secrete a variety of products such as lipids and proteins into both the donor and receiver compartments. These substances can result in a variable background in solutions and may interfere with solute quantitation. Even if a chromatographic method is used with fluorescence or UV detection, these products can still interfere with the separation unless specifically accounted for. 

All but one of the seven laboratories that recently participated in a intercomparison of niacin assays chose to use microbiological assays (42). The results from the lone HPLC determi-nation/UV absorbance determination were rejected due to lack of chromatographic resolution. 

Hantash, J., Bartlett, A., Oldfield, R, Denes, G., O Rielly, R., Roudiere, D., Menduni, S. (2006). Use of an on-line imprinted polymer pre-column, for the liquid chromatographic-UV absorbance determination of carbaryl and its metabolite in complex matrices,... 

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