Uniform binding energy change

Protein engineering (continued) differential and uniform binding energy changes 438,439 dissection of catalytic triad of serine proteases 450-452 domain structure analysis... 

Examples and consequences of uniform and differential binding effects are discussed in Chapter 15, section H. Uniform binding energy changes are effective in increasing kcJKM when [S] < KM, and this will increase the reaction... 

Like the uniform density model, it is simple, and has been used to understand the effect of changes in the nuclear parameters on binding energies and wave-... 

Figure 9.17 A represents [S] > Kj (when Km and Kj are similar, we can use Km here). This requires that the substrate drop in energy on binding to the enzyme, compared to its value in free solution, and so the substrate has to climb out of a well to reach the transition state. We can consider Figure 9.17 A as our starting point, and we want the enzyme to evolve into a more effective catalyst. The addition of another binding interaction that is equal in strength for both the substrate and the transition state (called a uniform binding change) does nothing to decrease the barrier associated with and thus it does nothing to accelerate the reac-...

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