Ultraviolet/visible light spectrophotometr

Spectrophotometry (the measurement of light absorption or transmission), is one of the most valuable analytical techniques available to biochemists. Unknown compounds may be identified by their characteristic absorption spectra in the ultraviolet, visible, or infrared. Concentrations of known compounds in solutions may be determined by measuring the light absorption at one or more wavelengths. Enzyme-catalyzed reactions frequently can be followed by measuring spectrophotometrically the appearance of a product or disappearance of a substrate. 

The remaining methods of analysis addressed in this chapter are instrumental methods of analysis. The first group of these to be addressed are spectrophotometric methods based on the absorption and anission of photons of electromagnetic radiation including visible light, ultraviolet radiation, and infrared radiation. 

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