Ultraviolet irradiation viral inactivation

Translation of the viral genome is required to bring about the inhibition of cellular protein synthesis. If the poliovirus genome is inactivated by ultraviolet irradiation prior to the infection of cells, no inhibition of cellular protein synthesis can be observed (11, 12), The OT treatment results in, among other alterations, the formation of uridine dimers in the ENA, thus blocking its translation, but presumably has little effect on the input viral capsid proteins. If cells are infected in the presence of cycloheximide (ll) or puron rcin, upon removal of the drugs, protein synthesis resumes at the control levels of uninfected cells for a... 

Another approach is the whole-killed or inactivated vaccine. These vaccines are derived from the pathogen itself, rather than a weakened form, as are live attenuated vaccines. Chemical (e.g., formalin, ether, and beta-propiolactone) or physical (e.g., heat, ultraviolet, and gamma irradiation) means, or combinations thereof, are used to inactivate the pathogen. Notable examples of this type of viral vaccine include the inactivated polio vaccine... 

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