Ultracentrifugation, analytical molecular weight

Analytical ultracentrifugation (AUC) Molecular weight M, molecular weight distribution, g(M) vs. M, polydispersity, sedimentation coefficient, s, and distribution, g(s) vs. s solution conformation and flexibility. Interaction complex formation phenomena. Molecular charge No columns or membranes required [2]... 

Human RBP has a molecular weight of 21,200 (Peterson, 1971a) to 21,300 (Raz et al., 1970), as determined by sedimentation equilibrium analysis in the analytical ultracentrifuge. The molecular weight calculated from the reported... 

Figure 3. Sedimentation diffusion equilbrium of 1 3 as measured in the analytical ultracentrifuge. A molecular weight of 6,500 was calculated using the equation shown in the figure.

With this relationship for all samples was calculated from ninh This M is used for evaluating the reaction data. The ultracen rifuge (u.c measurements were carried out in a Spinco model E analytical ultracentrifuge, with 0.4% solutions in 90% formic acid containing 2.3 M KCl. By means of the sedimenta- ion diffusion equilibrium method of Scholte (13) we determine M, M and M. The buoyancy factor (1- vd = -0.086) necessary for tSe calculation of these molecular weights from ultracentrifugation data was measured by means of a PEER DMA/50 digital density meter. 

Determination of a Polymer s Molecular Weight Distribution by Analytical Ultracentrifugation... 

A variety of analytical measurements can be made on biological samples during and after a centrifuge run. Most often, the measurements are taken to determine, molecular weight, density, and purity of biological samples. All analytical techniques require the use of an ultracentrifuge and can be classified as differential or density gradient... 

The purified hemagglutinin was homogeneous by gel filtration and immuno-electrophoresis, and in the analytical ultracentrifuge.63,560,561 A molecular weight of 79,000 was determined by the sedimentation equilibrium method561,569 (100,000 by sedimentation and velocity measurements,83 and 53,000 by gel filtration563). 

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