Tyvelose-specific antibodies

Fig. 6.2. Caco-2 epithelial cell monolayers cultured with T. spiralis L1 larvae in (A) the absence or (B) presence of 1 mg ml 1 rat monoclonal, tyvelose-specific antibody 9D4 (McVay etal., 2000). Monolayers were fixed and stained with trypan blue as described in ManWarren etal. (1997). (A) Serpentine trails of nuclei in dead cells are evident, revealing the paths travelled by larvae. (B) Tyvelose-specific antibody has inhibited the migration of the larva such that it is encumbered in cell debris and has pulled up a large area of the monolayer, creating a plaque (P). Bar = 50 urn. Photomicrograph prepared by C. McVay, TTUHSC, Lubbock, Texas. 

G., Garegg, P.J., Svensson, S., and Wallin, N.-H. (1979). Immuno-chemistry of Salmonella O-antigens. Specificity and cross-reactivity of factor 09 serum and of antibodies against tyvelose a-(l—3)-mannose coupled to bovine serum albumin. Int. Archs Allergy appl. Immun. 58, 11-19... 

Antibodies specific for tyvelose containing epitopes account for a large proportion of the immune response in Trichinella-infected patients.218... 

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