Tyrodes ringer saline

The composition of Tyrodes ringer saline for electroporation of embryos... 

Tyrodes ringer saline is prepared according to the formulation in Table 2. After preparation the pH of the solution is adjusted to pH 7.3 with 1M NaOH. The solution is sterilized with a 0.22-pm Millipore filter. The solution is stored at 4°C. 

Pick up the embryo using mouth-controlled transfer pipet and place into the drop containing Tyrodes ringer saline. 

Place a drop of Tyrode s Ringer saline over the platinum plate until it is completely immersed. Place a separate drop of PBl on the petti dish. 

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