Tyrode-albumin solution

Once the RBCs or platelets are harvested from the subject, there still exists some sample preparation prior to the measurement portion of the analysis. For example, in order to purify platelets, the collected blood is centrifuged at 500 g at 37°C for 10 min. The platelet-rich plasma (PRP) is decanted for the subsequent isolation of platelets. Platelets are then isolated from the PRP by centrifugation (15 min at 2000 g at 37 C) and washed three times in Tyrode-albumin solution (pH 7.4). The first wash contains heparin (2 U/mL) and apyrase (1 U/mL) the second wash contains only apyrase (1 U/mL) and the third wash contains Tyrode s solution without apyrase and heparin. 

In order to determine the blood compatibility of the plasma treated materials, platelet adhesion studies were conducted since platelet adhesion is one of the most important steps during blood coagulation on artificial surfaces. Blood samples were drawn from healthy canines (adult mongrel dog) which were not on medication. Platelet-rich plasma was obtained and the platelets were washed by albumin density gradient centrifugation in Hepes-Tyrodes buffer solution of pH 7.3 by a modification of Walsh and Griffin s... 

Test solutions of TNF-a (IDS Ltd.) were prepared in a Tyrode buffer containing 0.1% of bovine serum albumin, BSA, with final cytokine concentrations ranging from 0 to 1000 pg mL". Solutions (15 mL) were incubated at 25°C and shaken, (100 rpm) with 0.3 g of adsorbent for 6 hours. Aliquots of solutions taken at various times were analysed using ELISA kits (IDS Ltd.). 

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