Types Based on Chromatographic Modes

FIPLC columns can be categorized into four major modes (NPC, RPC, IEC, and SEC) in addition to other specialized modes (e.g., affinity, chiral, or specified applications).1,3,9 Since reversed-phase chromatography (RPC) is used in 70-80% of all FIPLC applications, RPC columns for small molecules are the focus of this discussion. 

Column type Typical i.d. (mm) Typical flow rate (mL/min) Void volume (mL) Typical sample loading 

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