Two-electrode voltage clamp

Schematic diagram illustrating a typical two-electrode voltage clamp setup for the study of currents of ligandgated ion channel receptors expressed In Xenopus oocytes. The oocyte is voltage clamped in the bath and drug-evoked currents are recorded (see text)...

Schnizler et al., 2003) permits the automation of both cRNA injection and two-electrode voltage clamp recordings from multiple oocytes in standard 96-well plates. Although the cost and maintenance of such an apparatus is likely to be restricted to industrial research environments, other automated oocyte perfusion systems have been described that would be suitable for smaller scale laboratories (e.g., Joshi et al., 2004). 

Figure 30-18 (A) K+ currents recorded from Xenopus laevus oocytes carrying cloned genes of Drosophila shaker K+ channels under two-electrode voltage-clamp conditions. Trace 1.4-IR was obtained from a cell expressing channels that lack the inactivation gate. Trace 1.4-IR + Pj2/ obtained from a cell expressing P subunits as well, shows rapid self-inactivation. (From Zhou et (B) Composite model of a voltage-dependent K+ charmel. The pore structure in the a subunit is represented by the...

Acetylcholine Binding Protein, Chicken a4 Subunit, Chicken (32 Subunit, Drosophila mdanogasterDal Subunit, Homology Modeling, Loop D, Neonicotinoid, Nicotinic Acetylcholine Receptor, Two-Electrode Voltage-Clamp... 

The use of site-directed mutagenesis combined with two-electrode voltage clamp electrophysiology revealed that G189D and G189E mutations markedly reduced... 

Figure 15.2. Drosophila larval neuromuscular junction system. A wandering third-instar larva is dissected open to reveal the ventral neuromusculature (see Figure 15.1). The peripheral nerve is severed and stimulated with a glass suction electrode. The muscle is recorded from in two-electrode voltage-clamp (TEVC) configuration. The postsynaptic excitatory junctional current (EJC) is recorded to assay synaptic transmission bottom inset). Top inset) Basis of synaptic transmission event being evoked by nerve stimulation and recorded via ion flux through muscle glutamate receptors.

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