Two-dimensional procedures involving isoelectric focusing

The third variation that exploits unsliced gels is based on the following principle the gel is, after isoelectric focusing, incubated with antiserum and the non-precipitated proteins are eluted. Further processing, e.g., staining and destaining is conventional. Methods that use slicing can be also modified in at least three different ways the individual sections can be tested by double gel diffusion, radial diffusion or immunoelectrophoresis. 

As a matter of fact any of the methods used in immunoelectrophoresis are applicable (see the corresponding section). 

---