Two-dimensional gel electrophoresis systems

Poehling reported a microscale two-dimensional gel electrophoresis system 25 years ago (Poehling and Neuhogg, 1980 Neuhoff, 2000). In that system, separation in the IEF dimension was performed in thin, gel-filled tubes. After IEF, the gel was transferred to a 3 cm x 3.5 cm polyacrylamide gel, where proteins were separated by SDS-PAGE. Several hundred components were resolved from a few micrograms of protein homogenate. 

Hartinger, J., Stenius, K., Hogemann, D. and Jahn, R. (1996) 16-BAC/SDS-PAGE a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal. Biochem. 124, 126-133. 

Hartinger, J., et al. (1996). 16-BAC/SDS-PAGE A Two-dimensional Gel Electrophoresis System Suitable for the Separation of Integral Membrane Proteins, Anal. Biochem. 240 126-133. 

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