Developing chambers twin trough

The twin-trough chamber (Fig. 43A) was not just developed to economize in mobile phase it also allows the layer to be impregnated as desired from the vapor... 

Ascending, one-dimensional development in a twin-trough chamber. 

Ascending, one-dimensional development in a twin-trough chamber with chamber saturation with the layer being preconditioned in the solvent-free half of the trough for 15 min after application of the sample. 

Ascending, one-dimensional development at 10 —12 °C in a twin-trough chamber with 5 ml cone, ammonia solution in the trough containing no mobile phase (chamber saturation 15 min). 

Ascending, one-dimensional multiple development method (stepwise technique, drying between each run) in two mobile phase systems in a twin-trough chamber without chamber saturation (equilibration 30 min at 20-22°C) at a relative humidity of 60 — 70%. 

Ascending, one-dimensional development at 10 —12°C in a twin-trough chamber, with 25% ammonia in that part not containing mobile phase. The chamber was equihbrated for 15 min before starting development. 

Ascending, one-dimensional development in a trough chamber with chamber saturatioiL After sample application the HPTLC plate was preconditioned for 30 min in the mobile phase-free trough of a twin-trough chamber (without filter paper lining). 

Ascoiding, one-dimensional development in a twin-trough chamber (Camag) with 5 ml ammonia solution (25%) in the trough free from mobile phase. Chamber saturation ca. 15 min development at 10—12°C. 

FIGURE 5.24 Developing chambers 20 X 20 cm for classical preparative purpose of some manufacturers (left to right) CAMAG twin-trough chamber and flat-bottom chamber. Desaga standard separating chamber, Analtech chamber. 

The twin-trough chamber. Figure 7.17, is the simplest of the HPTLC developing chambers [193]. It consists of a standard... 

Once the samples on the HPTLC plate have dried, the plate is inserted into a twin trough chamber such as the one shown in Fig. 13.4 for development. Approximately 10 ml of developing solvent is placed on both sides of the tank, and a piece of blotter paper is placed on the trough opposing the HPTLC plate. The blotter paper ensures a saturated environment for the plate to develop. 

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