Turnover number, succinate dehydrogenase

Turnover number, succinate dehydrogenase, 236-237 Tyrosine residues cytochrome hi reductase, 163 cytochrome c peroxidase, 355 glyceraldehyde-3-phosphate dehydrogenase, 21... 

The second type of reconstitution is demonstrated by the work of Bruni and Racker (179). These investigators reconstituted a succinate-ubiquinone reductase system from a King-type preparation of succinate dehydrogenase (2.6 nmoles flavin per mg protein), a preparation of cytochrome b (24-27 nmoles heme per mg protein) solubilized and purified with the use of bile salts and SDS, and mitochondrial or soybean phospholipids. The highest succinate-ubiquinone reductase activity achieved was 980 moles succinate oxidized per minute per mole of succinate dehydrogenase flavin. While this activity is only 10% of the turnover number of complex II, it is still quite appreciable for this type of reconstitution. Since the preparations of succinate dehydrogenase and cytochrome b used were not pure, it is not known what is the minimum number of components needed for reconstitution of succinate-ubiquinone reductase activity. The role and the exact nature of the b-type cytochrome used in these experi-... 

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