Trypanosoma cruzi GPIs

SCHEME 13.17 Completion of Vishwakarma s synthesis of the Trypanosoma cruzi GPI anchor. 

SCHEME 13.19 Completion of Nikolaev s synthesis of two Trypanosoma cruzi GPI anchors. 

N. Garg, R. L. Tarleton, and K. Mensa-Wilmot, Proteins with glycosylphosphatidylinositol (GPI) signal sequences have divergent fates during a GPI deficiency. GPIs are essential for nuclear division in Trypanosoma cruzi, J. Biol. Chem., 272 (1997) 12482-12491. 

R. A. Mortara, L. M. Minelli, F. Vandekerckhove, V. Nussenzwieg, and F. J. Ramalho-Pinto, Phosphatidylinositol-specific phospholipase C (PI-PLC) cleavage of GPI-anchored surface molecules of Trypanosoma cruzi triggers in vitro morphological reorganization of trypomastigotes, J. Eukaryot. Microbiol., 48 (2002) 27-37. 

The phosphoethanolamine in C6 position of Manlll is present in all GPIs and it serves as the Hnker to the protein. Additional phosphoethanolamines can be found at the C2 position of ManI (characteristic for mammalian GPIs) and C6 position of Manll. The presence of an aminoethylphosphonate at C6 position of GlcN is a unique feature of the GPIs from Trypanosoma cruzi [9]. 

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