Triple-stranded polyribonucleotide

Table 3 (section 3.2.2.2) contains melting temperatures of polyribonucleotide hetero-complexes (including base-modified complexes), both double and triple stranded. 

In order to elucidate the role of conformation of the immunogen, animals were immunized with complexes of polyribonucleotides (double- or triple-stranded) associated with MBS A. The antibodies obtained reacted specifically with double- or triple-stranded complexes and it was concluded that the specificity was determined by the macromolecular conformation of the immunogen (Nahon et al., 1967 Lacour et al., 1968 Michelson et al., 1971). Immunization of rabbits with DNA-MBSA or with double-stranded polynucleotide complexes adsorbed to MBS A ehcits antibodies belonging to the macroglobulin class (Stollar and Sandberg, 1966 Nahon-Merlin et al., 1973). 

In order to give DNA immunizing properties Plescia et al. (1964, 1965) complexed the material to MBS A. A certain number of polynucleotides such as denatured DNA, poly dAT, and tRNA associated with MBS A have been used as immunogens by these authors. This method of preparation has been used for other polynucleotides, particularly for single-stranded synthetic polynucleotides and the various double- and triple-hehcal complexes formed from such polyribonucleotides. It appears that secondary structure of these complexes is generally maintained on adsorption onto MBS A, although some of their characteristics could well be modified in this electrostatic interaction. 

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