Triglycerides ozonides

The ozonides of choline and ethanolamine phosphatides and triglycerides can be subjected to reduction with triphenylphosphine to yield the corresponding core aldehydes, and further derivatized to the 2,4-dinitrophenylhydrazones (DNP). The core aldehydes and their DNP derivatives can be separated by HPLC and characterized by various techniques, including EI-MS and TS-MS of positive and negative ions . See also Section VHI.E. 

Solvents. Hexane-diethyl ether mixtures are used as a rule in TLC. Hexane-diethyl ether (90 + 10) is suitable for the ozonides of the methyl esters of higher fatty acids and hexane-diethyl ether (80 + 20) for those of triglycerides [168]. 

It has been possible to show with the help of adsorption TLC, that isomers are formed when the methyl esters of unsaturated fatty acids are ozonised each of the isomers from methyl oleate has been isolated in a pure state [175]. Compounds which are not easily fractionated by argentation TLC, like as-methyl octadecenoate (methyl oleate) and mw5- mw5-methyl octadecadienoate (trans-trans methyl hnoleate ) or the corresponding triglycerides, have been separated as their ozonides [170]. This method is suitable, however, only for lipids with up to four double bonds. Ozone reacts with more highly unsaturated compounds to form short chain ozonides and undelfined side products [170]. 

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