Tri-N-acetylglucosamine

In addition to x-ray elucidation of the structure of the crystalline enzyme, the structure of a crystalline complex of lysozyme and tri(N-acetylglucosamine) was determined (Phillips, 1966). The trisaccharide occupied sites A, B, and C. Assuming that binding of a hexamer (adding hexose residues D, E, and F) would not change the conformation of the enzyme, the conformations of the substrate at... 

Thus far, ODMR measurements have been reported 9 -ioi,i78) only on the proteins bovine serum albumin, HLAD, lysoz mie, and lysoz3mie complexed with the inhibitor tri-N-acetylglucosamine (tri-NAG), and in these proteins only the tryptophan ODMR has been studied in detail. 

Peaks identified penta-O-methyl-mono-O-acetylmyoinositol derived from mono-linked myoinositol, 2,3,6-tri-O-methyl-1,4,5-tri-O-acetylglucitol derived from a 4-linked glucose, and 3,4,6-tri-O-methyI-l, 5,di-0-aeetyl-2-acetamido-2-N-methylglucitol derived from a terminal H-acetylglucosamine. The PMAA sample was chromatographed on a 1.5 m X 2 mm ID column packed with 3% OV-210 in a Finnigan automated GC/MS model 3300/6110. Temperature program 150° to 215°C at 6°C/min. 

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