Trematomus borchgrevinki

Fig. 1. The antifreeze glycoproteins have been numbered according to the order of their migration during electrophoresis. The diagram shows the electrophoretic pattern of mixed antifreeze glycoproteins of Trematomus borchgrevinki serum in borate buffer in acrylamide gel numbers refer to the eight different glycoproteins found in the serum. From Vandenheede (1972).

Fig. 2. Disc electrophoretic patterns of blood sera (1) man (2) king salmon (3) rainbow trout (4) Trematomus borchgrevinki, and (5) Dissostichus mawsoni. For D. mawsoni the most acidic component is the albumin, the next two bands the transferrins, and the antifreeze glycoproteins appear between the origin and the first band when the gel is stained for carbohydrate. From Komatsu et al. (1970b), reproduced with permission.

Fig. 3. Separation of serum proteins by gel filtration on Sephadex G-200. Elution was done with 0.1 M Tris-HCl (pH 8.0) containing 0.2 M NaCl. (A) Elution profile obtained from 12 ml of Trematomus borchgrevinki serum. (B) Elution profile obtained from 12 ml of human serum. Each tube contained 8 ml. The following abbreviations are used Alb., albumin Trans., transferrin 7-glob., 7-globulin o-/3 lip., a-fi lipoprotein. These areas apply also to (A), and areas I, II, III, and IV apply also to (B). From Komatsu et al. (1970b), reproduced with permission.

Fig. 4. Purification of antifreeze glycoproteins from Trematomus borchgrevinki serum. Sixty-six milliliters of T. borchgrevinki serum were dialyzed against 2.5 mM Tris at pH 9.6 and chromatographed on a DEAE-cellulose column (3.3 x 100 cm) equilibrated with the same buffer. The sample size was 13 ml per tube. Fraction I was eluted with the starting buffer, and fractions II and III were eluted with 50 mM Tris-HC1 at pH 9.6. Fraction IV was eluted with 0.2 M Tris-HCl at pH 9.6, and fraction V was eluted with 2 M Tris-HCl at pH 6.8. From DeVries et at. (1970), reproduced with permission.

Chemical Composition of Antifreeze Glycoproteins from Trematomus borchgrevinki and Dissostichus mawsoni ... 

Fig. 11. The effects of acrylamide gel concentration on the mobilities of glycoproteins isolated from Trematomus borchgrevinki. Rm is the relative mobility. From DeVries et al. (1970), reproduced with permission.

There have been studies on at least one dozen species of fishes in McMurdo Sound. Those most studied by Antarctic investigators are Trematomus borchgrevinki, T. bernacchii, and Dissostichus mawsoni. The characteristics of the fish are discussed elsewhere (8,12). 

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