Transmission electron microscopy positive staining

The size and shape of dendrimers have been estimated using transmission electron microscopy [14]. Producing better images of dendrimers may supply answers to fundamental questions such as whether dendrimers have truly uniform sizes, spherical shapes, and dense cores. The visualization of dendrimer assemblies would also be helpful to confirm the structure and morphology for many applications of dendrimers. The positively stained PAMAM dendrimers are shown in Fig. 2 for G10 to G5, respectively. The dendrimer molecules appear as dark objects on a light background of the amorphous carbon substrate and they are well separated from each other. The shapes of the stained molecules are spherical to a first approximation for G10 to G7, with some molecules showing... 

Fig. 12.2 The contracting areas within the embry-oid bodies (EBs) displayed molecular, structural, and functional properties of early-stage human cardiomyocytes. These properties include expression of cardiac-specific genes and transcription factors and the positive immunocytochemical staining for cardiac-specific proteins (e.g., ANP and cTnl). Transmission electron microscopy studies demonstrated the presence of an early sarco-...

---