Transient templates method

Coco, W.M. (2003) RACHITT gene family shuffling by random chimeragenesis on transient templates. Methods in Molecular Biology (Clifton, NJ), 231, 111-127. 

Coco, W. M., RACHITT Gene family shuffling by Random Chimeragenesis on Transient Templates. Methods. Mol.Biol., 2003. 231 pp. 111-127. 

Random Chimeragenesis on Transient Templates (RAC HITT) all generate library members are shuffled more crossovers than possible with PCR methods Coco, 2001... 

When hydrated chromium]III) chloride reacts with L739 in ethanol, the compound [Cr(L739)Cl2]Cl is formed (Eq. 3.51). Treatment of the latter with 2,6-dfp imder conditions of the transient template reaction mentioned above gives rise unexpectedly to [Cr(L742)(H20)2]Cl3-2H20, the structure of which was established by X-ray diffraction methods. In this compound the central atom is in a regular pentagonal-bipyramidal environment, with the axial positions occupied by water molecules. This is a rare example of a seven-coordinate macrocyclic complex of chromium(III) [133],... 

Figure 16.3 Computational template for the method of false transients.

The false-transient method can be applied to convective diffusion equations in a manner similar to that used for velocity profiles. Finite-difference approximations are written for the spatial derivatives. Second-order approximations can be used for first derivatives since they involve only the same five points needed for the second derivatives. The result is a set of simultaneous ODEs with (false) time as the independent variable. The computational template of Figure 16.3 is unchanged. The next two examples illustrate its application to problems where axial diffusion is negligible. Such problems are also readily solved by the method of lines as described in Chapter 8. Cases with significant axial diffusion are troublesome for the method of lines and require special boundary conditions for the method of false transients. They are treated in Section 16.2.4. 

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