Transgenic studies conformations

The conformation of plasmid DNA, namely supercoiled, open circular, or linear has been hypothesized to play a role in its transfection efficiency. While conventional thinking has generally indicated a preference for supercoiled DNA, at least one study has shown that the delivery of linear DNA results in prolonged transgene expression in vivo, due to con-catamerization (52). Other studies have shown that the conformation of DNA is irrelevant to the transfection efficiency in vitro and in vivo (53). 

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