Transgenic mice yeast

A transgenic mouse model expressing a PrP mutant without the N-terminal signal sequence (cytoPrP) conclusively showed that targeting of PrP to the cytosolic compartment can be neurotoxic [49, 68]. Further evidence for a toxic potential of cytosolically localized PrP was provided by additional mouse models [67, 69], by several mammalian cell culture models [49, 70, 71], and by a yeast model [72]. 

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