Transaminase-Catalyzed Reactions with Whole Cells

5 Transaminase-Catalyzed Reactions with Whole Cells 

Reaction scheme for the synthesis of L-isoieucine with whoie ceiis. 

SCHEME 29.15 Reaction cascade for the synthesis of chirai amino aicohois in whoie ceiis. 

The reaction is driven by ammonium and glucose feed to the media. The NADH was regenerated by cellular metabolism from a carbon source like glucose. 

2-Keto-3-methyl-valerate (KMV) was the least inhibitory substrate up to 40 mM and was therefore chosen for the synthesis of L-isoleucine. The best reaction yields and almost complete conversion of 100 mM KMV were obtained with 50 mM MOPS, 100 mM (NH )2S0, and a glucose/KMV ratio of 1 2. In this reaction the overall yield of 0.6g/gh was obtained. 

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