Trametes versicolor, production

SSF containing wheat bran and soybean as a substrate was chosen for the production of ligninolytic enzymes for Funalia trogii ATCC200800 [18] as it mimics the natural environment of the WRF and permits the concentration of dyes by absorption process prior to biological treatment [4, 38, 39]. It is possible to stimulate the yield of laccase activity of Trametes versicolor by using several agricultural wastes [40]. 

Birhanli E, Yesilada O (2006) Increased production of laccase by pellets of Funalia trogii ATCC 200800 and Trametes versicolor ATCC 200801 in repeated-batch mode. Enzyme Microb Technol 39 1286-1293... 

Lorenzo MD, Moldes D, Rodriguez Couto S, Sanroman A (2002) Improving laccase production by employing different lignocellulosic wastes in submerged cultures of Trametes versicolor. Bioresour Technol 82 109-113... 

BP Roy, MG Paice, FS Archibald, SK Misra, LE Misiak. Creation of metal-complexing agents, reduction of manganese dioxide, and promotion of manganese peroxidase-mediated Mn(III) production by cellobiose quinone oxidoreductase from Trametes versicolor. J Biol Chem 269(31), 19745-19750, 1994. 

K Addleman, T Dumonceaux, MG Paice, R Bourbonnais, F Archibald. Production and characterization of Trametes versicolor mutants unable to bleach hardwood kraft pulp. Appl Environ Microbiol 61(10) 3687-3694, 1995. 

This chapter describes our recent advances on the utilization of polymer-modified laccase complexes in aqueous systems towards the oxidation/polymerization of naturally hydro-phobic steroidal compounds, Equilin (EQ) and 17-P-estradiol (P-EST). We elucidate the kinetic and synthetic aspects of the process with the model compoimd 5,6,7,8-tetrahydro-2-naphthol (THN). The nano-reactor system is composed of linear poly(ethylene oxide)-dendritic poly(benzyl ether) diblock copolymer (G3-PE013k) and laccase isolated from Trametes versicolor. Other advantages of the complex in comparison to the native enzyme are its recyclability, enhanced stability, activity, and overall simplicity in product harvesting and isolation. A principle of action of the complex is suggested based on these findings and is further supported by the biphasic solid-liquid nature of the reaction medium, which exhibits continuous influx of starting material and steady solid product expulsion. Comparative experiments with linear-linear poly(styrene)-Woc -poly(ethylene oxide) copolymer under identical conditions do not evince formation of a... 

Figure 25. The sequential photocatalytic treatment of the effluent previously treated with Trametes Versicolor. The irradiation time used was 60 min. Reprinted from, Aura M. Pedroza, Rodolfo Mosqueda, Nicolas Alonso-Vante and Refugio Rodriguez-Vazquez, Sequential treatment via Trametes versicolor and UV/Ti02/RuxSey to reduce contaminants in waste water resulting from the bleaching process during paper production, Chemosphere 67 (2007) 793, Copyright (2007), with permission from Elsevier.

S. (2012) Engineering and production of laccase from Trametes versicolor in the yeast Yarrovvia lipolytica. Bioresour. Technol, 125, 267-274. 

Treatment with the enzymes peroxidase and laccase, obtained from the ligninolytic fungus Trametes versicolor, has been shown to increase the maximum ethanol productivity in a hemicellulose hydrolysate (Palmqvist and Hahn-Hagerdal, 2000). To detoxify phenolic inhibitors, laccase from T. versicolor was expressed in S. cerevisiae. The laccase expressing strain had the ability to convert coniferyl aldehyde at a faster rate than a control strain not expressing laccase, which enabled faster growth and ethanol fermentation in the presence of coniferyl aldehyde (Larsson et al., 2001). 

Maize bran and nejayote were kindly provided by tortilla-making industry in Mexico. Laccase (benzenediol oxygen oxidoreductase, E.C.1.10.3.2) was obtained from Trametes versicolor. All chemical products were purchased from Sigma Chemical Co. (St Louis, MO, USA). 

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