Total fresh weight, relationship

Development of model. Experiments were conducted to develop a model for predicting shoot-plus-root fresh weight (PSRFW) from total fresh weight (TFW). This seemed reasonable because of the apparent relationship between shoot fresh weight versus shoot length, and... 

Intact plastids were isolated from early, early to mid and mid to late cotyledon embryos (1.5, 2.5 and 3.5 mg embryo fresh weight) of B. napus cv. Topas (according to [1]). Isolated plastids were incubated with " C-labelled metabolites and the incorporation of C into fatty acids determined [1]. The activities of enzymes were measured using standard assays [1] optimized for plastid extracts. The proportions of glycolytic enzyme activities attributable to the plastid were determined by adding increasing concentrations of cytosol (supernatant) back to a plastid pellet. The plastidial activity of each enzyme was then calculated from the relationship between its total activity and that of a cytosolic and plastidial marker enzyme essentially as previously described [3]. Short term assays for the rate at which isolated plastids take up Relabelled metabolites were performed using silicone oil filtration [4]. 

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