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TLC Developing Chambers for Horizontal Development

With developing chambers for horizontal development, the chamber type again determines the price. [Pg.90]

Developing chambers for horizontal development were originally conceived as sandwich chambers (S-chambers) in which the plate, with the layer imdemeath, is placed above the counter-plate at a distance of 0.5 mm. Without this counter-plate, a filter paper soaked in solvent can be placed at the bottom of the chamber, enabling development to be performed in a saturated chamber. Chromatography can be performed with only a few ml solvent in horizontal chambers, which is, of course, economic and reduces disposal problems. However, the question arises how much of a four- or five-component solvent system is actually used if only 2 ml is consumed in the development process in the 5 x 5 cm horizontal chamber  [Pg.91]


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