Titration of BSA with TNS at pH 3 and

To a cuvette containing 1.5-2 pM bovine serum albumin solution, add equal volumes of TNS solution so that you have 0.3-15 pM TNS in the cuvette. For each TNS concentration 

Measure the fluorescence intensities at 335 nm at the different TNS concentrations, and correct them first for the dilution then for the inner filter effect. Finally, plot the fluorescence intensity at 335 nm as a function ofTNS concentration. What do you observe  

For each TNS concentration, measure the intensity variation A I, and then plot 1/Af as a function of 1/[TNS]. By extrapolating to 1/[TNS] = 0, we can calculate AImax. Is AImax the same for both pHs Explain. Determine the apparent value of the association constant J Ca of the TNS-BSA complex. Are the association constants equal Explain. 

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